The Pugh lab is focused on the application of genome sequencing analysis as a routine clinical test and creating a bridge from bench to bedside.  We are currently focused on understanding clinical implications of clonal shifts in cancer and non-cancerous cell populations during treatment, most recently using cell-free DNA, immune repertoire, and single cell RNA-seq sequencing. We routinely perform genome analysis of surgical specimens, serial biopsies, blood samples and circulating tumor DNA collected from patients diagnosed with a wide range of cancers. Dr. Pugh also supports clinical testing as a molecular geneticist and Director of the Joint Genomics Program of the University Health Network and Ontario Institute for Cancer Research.